Compatible with chemiluminescent substrates and fluorescent secondary antibodies. Western blotting: detection of the two unstained bands via the detection method used for the target protein.Western blot confirmation-detect the two unstained proteins (30 kDa and 80 kDa) using the detection system for the target protein.A ready-to-use formatno mixing, heating, or reducing required. Nine polypeptides that resolve into sharp, tight blue bands in the range of 4200 kDa (depending upon the buffer system) An excellent indicator for judging Western transfer efficiency. Seeblue plus 2 Prestained Standards (Invitrogen) were used as. Fluorescent visualization-detect the 10 stained proteins using the 670 nm red laser or 700 nm channel of a fluorescence imager The SeeBlue Pre-Stained Standard can be used with NuPAGE, tris-glycine and tricine gels. Protein concentration was determined by the BCA.The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.Ĭompare and view all other protein standards and ladders › Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading. Prestained standards were run on a Bio-Rad CriterionTM 420 precast gel in Tris-glycine-SDS running buffer at 200 V for 45 min. IBright Prestained Protein Ladder contains twelve recombinant proteins, ten (11 to 250 kDa) that are blue-stained and fluor-labeled for direct and near-IR fluorescent visualization and protein sizing, and two (30 kDa and 80 kDa) that are unstained and contain IgG binding sites to bind the primary and secondary antibodies used for chemiluminescent and fluorescent detection of the target protein. Protein ladders and standards for SDS-PAGE, western blots, and isoelectric focusing (IEF) Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. Description L’étalon précoloré SeeBlue Plus2 contient 10 protéines : huit colorées en bleu et deux de couleurs contrastantes pour identifier les bandes plus facilement. Which SeeBlue Plus2 Pre-Stained Std contains 10 proteins: eight blue-dyed and two with contrasting colors providing easier group identification. Invitrogen SeeBlue Plus2 Pre-stained Protein Standard.
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